P050 Intraperitoneally injected 3-dimensional cultured tonsil-derived mesenchymal stem cells in murine colitis model form aggregates with mouse immune cells: A preliminary study

Abstract Background 3-dimensional (3D) cultured tonsil-derived mesenchymal stem cells (TMSCs) showed higher therapeutic efficacy in murine colitis model than conventional 2D-cultured TMSCs via increased anti-inflammatory cytokine expression. Here, we evaluated the in-vivo localization and formation of 3D-cultured using electron microscopy. Methods Chronic mouse was induced by oral administration 1.5% dextran sulfate sodium for 30 days. injected intraperitoneally on day 6 16 after inducing mice. TMSC as a spheroid 24 hours before intraperitoneal injection. On 31, mice were euthanized immunofluorescence analysis. Moreover, characterization Results In vitro spheroids measured to have an average size 182.4 μm 1, 169.4 2, 154.1 3; however, gradually decreased 2 days culture. treated with TMSCs, there no detectable at time sacrifice. white variable sized spherical aggregates observed. These attached peritoneal cavity including omentum mesentery. fluorescence microscopy, these stained human nuclear antigen antibody, indicating that transplanted formed cluster peritoneum even 14 covered lymphocytes. inside spheroids, suspected cell mesothelial distributed. Conclusion Intraperitoneally found Based results current study, functional studies evaluating role therapy, cell-to-cell communication between immune cells, are needed.